Apoptosis Methods in Pharmacology and Toxicology: Approaches by Myrtle A. Davis

By Myrtle A. Davis

Univ. of Maryland, Baltimore. Discusses laser scanning cytometry for the research of apoptosis, tissue-based equipment of quantification for stories utilizing animal versions, and useful assays and ELISA strategies. DNLM: Apoptosis--physiology.

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Smith, C. L, Nechushtan, A, Xi, X. , and Youle, R. J. (1997) Movement of Bax from the cytosol to mitochondria during apoptosis. J. Cell Biol. 139(5), 1281–1292. Mahajan, N. , Gordon, G. , and Herman, B. (1998) Bcl-2 and Bax interactions in mitochondria probed with green fluorescent protein and fluorescence resonance energy transfer [see comments]. Nat. Biotechnol. 16(6), 547–552. Qin, Z. , Kikly, K. , Kegel, K. , and DiFiglia, M. (2000) Pro-caspase-8 is Predominatly localized in mitochondria and released into cytoplasm upon apoptotic stimulation.

In 32D cells induced to undergo apoptosis through interleukin-3 (IL-3) withdrawal, total [Ca2+]i remained unaltered, although a significant subcellular redistribution of Ca2+ from the cytoplasm to the mitochondria occurred, which could be inhibited by Bcl-2 expression (52). Some Ca2+-sensitive fluorochromes (Rhod-2 and derivatives) are reported to selectively partition into mitochondria and may be capable of detecting a local change in mitochondrial calcium. Fluorescence microscopy can determine the specific subcellular compartment in which an alteration of [Ca2+]i takes place through co-localization of calcium probe fluorescence with the appropriate organelle-specific markers.

Am. J. Physiol. 276(1 Pt 1), G1–G6. 4. Warny, M. and Kelly, C. P. (1999) Monocytic cell necrosis is mediated by potassium depletion and caspase-like proteases. Am. J. Physiol. 276(3 Pt 1), C717–C724. 5. Raffray, M. and Cohen, G. M. (1997) Apoptosis and necrosis in toxicology: a continuum or distinct modes of cell death? Pharmacol. Ther. 75(3), 153–177. 6. Cotran, R. , and Robbins, S. L. (1999) Robbin’s Pathological Basis of Disease. W. B. Saunders, Philadelphia. 7. , and Kroemer, G. (1998) The central executioners of apoptosis: caspases or mitochondria?

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