Advances in Applied Microbiology, Vol. 35 by Saul L. Neidleman (ed.), Allen I. Laskin (ed.)

By Saul L. Neidleman (ed.), Allen I. Laskin (ed.)

Meant for researchers in utilized microbiology and environmental engineers, this publication covers such subject matters as environmental overview of biotechnological procedures and microbial changes of haloaromatic and haloaliphatic compounds.

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1982). , 1947). However, the enzyme purity was -150% with the former system. , 1947). 7. A mechanically agitated system recovered -8% more enzyme than 38 B. K. I’ONSANE AND M. V. , 1982). 8. The moldy fermented solids are subjected to extraction immediately at the end of the fermentation or are air or vacuum dried. The dried bran can be stored for several months without any appreciable decrease in enzyme titer. It may be used as such, or the enzyme may be extracted whenever required. A survey of literature on the methodology adopted by various workers for extraction of bacterial a-amylase from bacterial bran produced in the SSF technique indicates that it is similar to the trends just described.

1960). I. AUTOCLAVING The moist solid medium requires cooking for physical modification of solid particles, softening and partial hydrolysis of starches and proteins present in the solids, as well as sterilization for killing the contaminant microbial cells and spores. , 1985). Negligible work on this subject was carried out in the case of a-amylase production by bacterial cultures in the SSF technique, and consequently parameters were either selected arbitrarily or based on practice followed in fungal SSF processes.

For example, too much of the moistening agent was reported to make the WB less suitable for bacterial growth and enzyme production LU-AMYLASE PRODUCTION BY SOLID-STATE FERMENTATION 27 TABLE V WATER-SOLUBLE NIJTRIENTS USEDALONG WITH WHEATBRAN Supplementary nutrient Urea Concentration (% or ratio) 1 g N per liter Reference Qadeer et al. (1980) diluent Casein Soybean extract Liquefied starch (by bacterial a-amylase) Ethanol (NH4lzHPO4 MgSO,. 17) CaClz . 5 Park and Rivera (1982) Park and Rivera (1982); Ramesh and Lonsane (1987b).

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